Casein peptone, tryptic digest 10.00 g;
Yeast extract 5.00 g;
Meat extract 5.00 g;
Bacto Soytone 5.00 g;
Glucose 10.00 g;
K2HPO4 2.00 g;
MgSO4 x 7 H2O 0.20 g;
MnSO4 x H20 0.05 g;
Tween 80 1.00 ml;
NaCl 5.00 g;
Cysteine-HCl x H2O 0.50 g;
Salt solution (see below) 40.00 ml;
Resazurin (25 mg/100ml) 4.00 ml;
Distilled water 950.00 ml.
Adjust pH to 6.8



Salt solution:
CaCl2 x 2 H2O 0.25 g
MgSO4 x 7 H2O 0.50 g
K2HPO4 1.00 g
KH2PO4 1.00 g
NaHCO3 10.00 g
NaCl 2.00 g
Distilled water 1000.00 ml