I. Yeast extract 6 g Glucose 5 g CaCl2.2H2O 0.25 g MgSO4.7H2O 0.5 g (NH4)2SO40.2 g KH2PO4 3 g Distilled water 500 ml pH 4.0 Sterilize at 115°C for 15 min.

II. Trace element solution* 1 ml

III. Agar 30 g Distilled water 500 ml Sterilize at 121°C for 15 min.

*Trace element solution: ZnSO4.7H2O 0.1 g MnCl2.4H2O 0.03 g H3BO3 0.3 gCuCl2.6H2O 0.2 g CaCl2.2H2O 0.01 g NiCl2.6H2O 0.02 g Na2MoO4.2H2O 0.03 gDistilled water 1000 ml Sterilize at 121°C for 15 min.

Mix together I - III and distribute the medium aseptically into sterile tubesor Petri dishes.