Gram-negative, motile, rod-shaped bacterial strains (designated 4F2T and Kf) were isolated from decaying tissues of various deciduous tree species. Phylogenetic analyses based on their 16S rRNA gene sequences showed that the novel isolates belong to the genus Brenneria and showed highest (98.3 %) sequence similarity to Brenneria goodwinii . Isolate 4F2T formed a separate branch on the phylogenetic tree based on concatenated sequences of four housekeeping genes or whole genome sequences, clearly separate from Brenneria goodwinii , suggesting that novel isolates should belong to a novel species. Orthologous average nucleotide identity scores and in silico DNA–DNA hybridization values between isolate 4F2T and type strains of other species in the genus Brenneria were less than 85 and 30 %, respectively, significantly lower than the species boundary cut-off values (95 and 70 %). A negative reaction for β-galactosidase, the ability to use dextrin and maltose as carbon sources, and an inability to use lactose are the main phenotypic characteristics that can be used to differentiate the novel isolates from B. goodwinii . Based on phenotypic and genotypic characteristics, isolates 4F2T and Kf belong to a novel species of the genus Brenneria , for which the name Brenneria bubanii sp. nov. is proposed. The type strain is 4F2T (=NCAIM B 02661T=LMG 32183T). https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem...