Yeast extract 1.0 g, Soil extract (see below) 200.0 ml, Mannitol 10.0 g, Agar 15.0 g, Distilled water 800.0 ml. Adjust pH to 7.2
Soil Extract: Air-dried garden soil 77.0 g, Na₂CO₃ 0.2 g, Distilled water, 200.0 ml.
Autoclave for one hour at 121 C. Filter through paper before using in media.