Glucose 10.00 g (NH4)2SO4 3.00 g KH2PO4 0.50 g MgSO4 x 7 H2O 1.00 g KCl 0.10 g Ca(NO3)2 x 4 H2O 18.00 mg FeSO4 x 7 H2O 0.01 mg Agar 15.00 g Distilled water 1000.00 ml In liquid medium, the pH should be adjusted to 3.5 with H2SO4. In solid medium, the pH should be adjusted to 4.5 with H2SO4 after autoclaving the medium. Sterilize the glucose and the basal solution separately.